The Allen Institute for Brain Science is a 501 (c) (3) nonprofit ...

www.aperio.com

...Brain Atlas Data Production Processes were used for the ISH procedures, with the exception of the proteinase K concentration used. Proteinase K was optimized at one cycle of 10 minutes at 0.0096 U/ml, which represents a compromise between...

http://www.aperio.com/pathology-events/Scientific%20Presentations/Allen_Institute_White_Paper.pdf

Proteinase K immobilized on matrix F7m, 1 column

Proteinase K immobilized on matrix F7m, 1 column

http://www.bocascientific.com/popup_image.php?pID=817&osCsid=a74924e6a1758c2da9712ba5b6167818

Cleaning Up DNA Samples

www.dddmag.com

...in DNA preparation kits must be as efficient as possible. And this includes efficient lysis conditions, the use of proteinase K in denaturation buffers to reduce PCR inhibition by proteins in the sample, and the use of carrier RNA to improve recovery...

http://www.dddmag.com/Feature-Cleaning-Up-DNA-Samples.aspx

Proteinase K

Proteinase K Product Proteinase K - MBI Description Produced by the fungus Tritirachium album...carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine pro-tease. Concentration Approximately...

http://www.cinnagen.com/Proteinase_K.htm

Extracellular K+ concentration controls cell surface density of IKr in rabbit hearts and of the HERG channel in human cell lines

www.jci.org | Sep 1, 2009

...after 32–36 hours of culture of proteinase K–treated HERG-HEK cells (PK...live cells with externally applied proteinase K. Several investigators, including...HERG-HEK cells with external proteinase K completely removes the cell surface...

http://www.jci.org/articles/view/39027

Autophagy regulates adipose mass and differentiation in mice

www.jci.org | Nov 5, 2009

...visual counting of individual cells. Immunohistochemistry. Five-micrometer paraffin sections were treated with proteinase K for antigen retrieval. Sections were stained with an F4/80 antibody using the BioModule Immunohistochemical Staining...

http://www.jci.org/articles/view/39228

Tyrosine and serine phosphorylation of {alpha}-synuclein have opposing effects on neurotoxicity and soluble oligomer formation

www.jci.org | Nov 5, 2009

...transgenic flies. Nor was there any detectable increase in proteinase K resistance, a typical feature of fibrillar protein...CrossRef PubMed Neumann, M., et al. 2002. Misfolded proteinase K-resistant hyperphosphorylated alpha-synuclein in...

http://www.jci.org/articles/view/39088

Expression of human BRCA1 variants in mouse ES cells allows functional analysis of BRCA1 mutations

www.jci.org | Oct 3, 2009

...buffer (1% SDS, 0.1 M NaHCO 3 ) and then reverse cross-linked in a 65°C water bath for 5 hours followed by proteinase K treatment for 2 hours. The final DNA was purified by phenol/chloroform extraction and ethanol precipitation with 1...

http://www.jci.org/articles/view/39836